Resting membrane potential can be defined as the potential difference that results from the separation of charges along the plasma membrane of a neuron or other excitable cell. A resting neuron is not conducting a nerve impulse. The plasma membrane of a resting neuron
All living cells have an electrical charge difference across their plasma membranes due to ionic imbalances, the inside of the cell being more negative than outside. This difference in charge gives rise to an electrical voltage gradient across the membrane, which can be measured.