Objectives of Transmission of Genetic Material In Bacteria

Multiple Choice Questions (MCQs) ‘

  1. The transfer of genetic material between bacteria through cell-to-cell contact is called bacterial. (a) Conjugation (b) Transformation(c) Transduction (d) None
  2. Episome is a:

(a) Bacteria       (b) Plasm id      (c) Virus           (d ) Cell

  1. Which of the followings has pilin gene?

(a) tra               (b) trb              (c) trc               (d) trd

  1. A complex of proteins creates a nick in plasmid. It is:

(a) Plasinid       (b) relaxosome (c) Polymer:Ise (d) Epimerase

  1. The transfer of entire gene of E.Coli takes minutes
    about:

(a) 14               (b) 16               (c) 30               (d) 34

  1. The process by which bacterial DNA is moved from one bacterium to another by a virus is: (a) Conjugation (b) Transformation(c) Transduction(d) None
  2. Restriction transduction is caused by:(a) E.Coli     (b) Retrovirus (c) Polio virus (d) None
    1. Transduction was discovered by:

    (a) Griffith   (b) Lederberg (c) Tatum      (d) De Varies

    1. Transformation was discovered by:

    (a) Griffith   (b) Lederberg (c) Tatum      (d) De Varies

    1. The compound used for artificial competence is:

    (a) 1-120     (b) CaCI,     (c) N113           (d) Alcohol

    Answers:

    I. (a) 2. (b) 3. (b) 4. (b) 5. (c) 6. (c)    7. (b) 8. (b)

    9. (a) 10. (b)

    Fill in blanks


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    1. The transfer of genetic material between bacteria through cell-to­cell contact is called bacterial  _________
    2. __________ is a plasmid of about I 00 kb length that can integrate  itself into the bacterial chromosome by genetic recombination.
    3. The_____ locus has pilin gene and regulatory genes.
    4. A complex of proteins called the _____ creates a nick in
      one plasmid DNA strand at the origin of transfer, or oriT.
    5. In the F-plasm id system the relaxosome consists of proteins Ira!,TraY, TraM, and the integrated host factor__
    1. Some culture of cells contains non-integrated F plasmids. These are responsible for the_ of chromosomal gene transfer.
    1. _______ is the process by which bacterial DNA is moved
      from one bacterium to another by a virus.
    2. Transduction was first discovered by _____ in 1952.
    3. Generalized transduction occurs in____ of phage virus.
    4. Specialized transduction occurs in cycle of phage
      virus.
    5. Ans:

    1. conjugation        2. Episome                3. Ira    

    4. relaxosome       5. 1HF                         6. lowfrequency.

    7. Transduction     8. Joshua Lederberg 9. lytic cycle

    10. Lysogenic

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