Objectives of Geneticengineering Biotechnology

Multiple Choice Questions (MCQs)

1. Which of the following restriction enzymes is used
   extensively:

(a) Type!           (b) Type II         (c) Type III        (d) Type IV

2. Which of the followings is DNAase?

(a) Type I          (b) Type II         (c) Type III       (d) Type IV

3. The various fragments of a specific DNA are separated into different bands of distinct molecular weights by:

(a) Alcohol         (b) agarose gel (c) Southern bolt(d) none

5. Specific sequences of bands can be identified by:

(a) Alcohol        (b) agarose gel (c) Southern bolt(d) Sonfication

6. The method used to generate small DNA fragments by
   mechanical shearing:

(a) Alcohol         (b) agarose gel (c) Southern bolt(d) Sonfication

7. Transformation of a cell with DNA from a virus is:

(a) Conjugation (b) transfection (c) Ligation          (d) None

8. Polyethylene glycol-induced DNA uptake in bacterial protoplasts is done in:

(a) E.coli           (b) Bacillus        (c) Phage          (d) Virus

9. Cosmids are:

(a) E.coli           (b) Plasmids      (c) Phage        . (d) Virus

10-The vectors used as vector in animals are:

(a) E.coli           (b) Plasmids      (c) Phage          (d) SV40
Answers:

I. (b) 2. (b) 3. (b) 4. (c) 5. (c) 6. (d) 7. (b) 8. (b) (b) 10. (d)

Fill in the blanks

1. Restriction ____ break the high-molecular-weight DNA
   into fragments.
2. ___ different classes of restriction enzymes are present.
3. Only type _________ restriction endonucleases are used
   extensively in the manipulation of DNA
4. The various fragments of a specific DNA are separated into different bands of distinct molecular weights by ____ gel electrophoresis.

5. Specific sequences of these bands can be identified by a technique called _____ blotting.

6. Other source of DNA is synthesis of_____________ DNA (cDNA). It is synthesized in test tube artificially by joining nucleotides.
7. Cohesive ends can also be created by the addition of synthetic

__________ to blunt-ended DNA molecules.

8. Transformation of a cell with DNA from a virus is called_______.

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