Objectives of Geneticengineering Biotechnology
Multiple Choice Questions (MCQs)
- Which of the following restriction enzymes is used
extensively:
(a) Type! (b) Type II (c) Type III (d) Type IV
- Which of the followings is DNAase?
(a) Type I (b) Type II (c) Type III (d) Type IV
- The various fragments of a specific DNA are separated into different bands of distinct molecular weights by:
(a) Alcohol (b) agarose gel (c) Southern bolt(d) none
- Specific sequences of bands can be identified by:
(a) Alcohol (b) agarose gel (c) Southern bolt(d) Sonfication
- The method used to generate small DNA fragments by
mechanical shearing:
(a) Alcohol (b) agarose gel (c) Southern bolt(d) Sonfication
- Transformation of a cell with DNA from a virus is:
(a) Conjugation (b) transfection (c) Ligation (d) None
- Polyethylene glycol-induced DNA uptake in bacterial protoplasts is done in:
(a) E.coli (b) Bacillus (c) Phage (d) Virus
- Cosmids are:
(a) E.coli (b) Plasmids (c) Phage . (d) Virus
10-The vectors used as vector in animals are:
(a) E.coli (b) Plasmids (c) Phage (d) SV40
Answers:
I. (b) 2. (b) 3. (b) 4. (c) 5. (c) 6. (d) 7. (b) 8. (b) (b) 10. (d)
Fill in the blanks
- Restriction ____ break the high-molecular-weight DNA
into fragments. - ___ different classes of restriction enzymes are present.
- Only type _________ restriction endonucleases are used
extensively in the manipulation of DNA - The various fragments of a specific DNA are separated into different bands of distinct molecular weights by ____ gel electrophoresis.
- Specific sequences of these bands can be identified by a technique called _____ blotting.
- Other source of DNA is synthesis of_____________ DNA (cDNA). It is synthesized in test tube artificially by joining nucleotides.
- Cohesive ends can also be created by the addition of synthetic
__________ to blunt-ended DNA molecules.
- Transformation of a cell with DNA from a virus is called_______.
Ans:
I. endonucleases 4. agarose 7. DNA linkers |
2. Three
5. Southern 8. transfection |
3.11
6. complementary |
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