Objectives & Fill in the blanks of Molecular Genetics

Multiple Choice Questions (MCQs)

  1. The model of DNA replication in which one strand is
    conserved and other is synthesized is:

(a) Conservative (b) Semi-const rvative(c) Dispersive (d) None

  1. In semi-conservativt model the strands separated and acts as:

(a) Enzyme    (d) Tool         (c) Model      (d) Finger prints

  1. The semi consm vative model of DNA was proved by:

(a) P.A. Levene                  (b) Meselson – Stahl

(c) F. Miescher                          (d) Watson and Crick

  1. The enzyme which attaches the fragments of DNA is:

(a) DNA polymerase I               (b) Primase

(c) Ligase                                 (d) DNA polymerase II

  1. The true replicating enzyme is:

(a) DNA polymerase III             (b) Ligase

(c) Primase                               (d) DNA polymerase 11

  1. The enzyme which play a supporting role in DNA replication is:

(a) DNA polymerase I               (b) Pirmase

(c) Ligase                                 (d) DNA polymerase II

  1. The DNA polymerase only add nucleotide to the end:

(a) 2                (b) 3                 (c) 4                 (d) 5

  1. The strands in which elongates towards replication fork is: (a) Leading strand(b) Lagging strand(c) (a) and (b) (d) None
  2. The length of Okazaki fragments is:

(a) 100 -200 nucleotide              (b) 200-300 nucleotide

(c) 300-400 nucleotide               (d) 400-500 nucleotide

  1. Okazaki fragment is a:

(a) RNA             (b) DNA            (c) Protein         (d) Enzyme

  1. Okazaki fragments are synthesize by:

(a) DNA polymerase 1               (b) Pirmase

(c) Ligase                                 (d) DNA polymerase III

  1. The synthesis of protein is called: (a) Replication (b) Transcription(c) Translation (d) None
  2. The synthesis of RNA from DNA is: (a) Transcription(b) Replication (c) Translation (d) None
  3. Anticodons are present on:

(a) mRNA         (b) rRNA           (c) tRNA            (d) non

  1. Codes are present on:

(a) mRNA         (b) rRNA           (c) tRNA            (d) non

  1. The strand which is transcribed during transcription is:

(a) Leading strand                     (b) Antisense strand

(c) Sense strand                        (d) Lagging strand

  1. mFtNA is synthesized by:

(a) RNA Polymerase I                (b) RNA Polymerase II

(c) RNA Polymerase III             (d) None of the above



  1. Transcription starts at:

(a) Operator      (b) Promoter (c) Enhancer (d) none


1. (b) 2. (c) 3. (b) 4. (c) 5. (a) 6. (d) 7. (b) 8. (a)
9. (a) 10. (b) 11. (d) 12. (c) 13. (b) 14. (c) 15. (a) 16. (b)
17. (b) 18. (b)

Fill in blanks

  1. The Watson – Crick Model suggests, that   is the basis of copying the genetic information.
  2. In semi conservative replication the strands of the duplex

separate. Each strand acts a_____________

  1. The complex process of DNA replication is catalyzed by DNA

____________ and other enzymes.

4-The enzyme___________ synthesiz.: an RNA primer.

5-Series of short segments of DNA are synthesized. These segments are connected later on. These segments are called__________.

6-The synthesis of inRNA from the DNA is called ___________

7-The RNA polymerase enzymes synthesize RNA from

__________ direction.

8-RNA polymerase has one subunits called_____________

9-The DNA strands open up at the place where enzyme.is attached

with the template strand. It forms_________________

10-The initiation codon is__________

11-Three codons UAA. UAG and UGA do not code for any amino

acid. So they are known as__________

12-The synthesis of protein from mRNA is called _______________

13-The tRNA molecules possess complementary three nucleotide

sequence. It is called__________

I. Complimentary 2. model 3. polymerase 4. primase

5. Okazaki       6. transcription7. 5 to 3      8. sigma

9. fork             10. AUG          11. termination12. translation

13. anticode   14. Initiation 15. releasing factor

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