Genetic engineering Biotechnology – Definition & Key points

TERMS DEFINITIONS
Genetic engineering The process of manipulating genes. usually

outside the organism’s normal   reproductive

process    is   called    genetic  engineering  or
genetic modification (GM) or gene splicing.

Sonification It is another method used to generate small DNA fragments by mechanical shearing.
Southern blotting Specific sequences’ of these bands can be

identified   by  a  technique  called                 Southern
blotting.

Transfection Transformation of a cell with DNA from a virus is called transfection.
Cosmids
Plasmids  have  also  been  constructed                that

contain the phage cos DNA site and ColE I

DNA segments. Cos DNA is required for


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packaging  into the  phage particles.ColE1

DNA    segments     required    for   plasmid

replication These  plasmids  are called  as
cosmids.


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