Genetic engineering Biotechnology – Definition & Key points

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TERMS

DEFINITIONS

Genetic engineering

The process of manipulating genes. usually

outside the organism’s normal reproductive

process is called genetic engineering or
genetic modification (GM) or gene splicing.

Sonification

It is another method used to generate small DNA fragments by mechanical shearing.

Southern blotting

Specific sequences’ of these bands can be

identified by a technique called Southern
blotting.

Transfection

Transformation of a cell with DNA from a virus is called transfection.

Cosmids

Plasmids have also been constructed that

contain the phage cos DNA site and ColE I

DNA segments. Cos DNA is required for

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packaging into the phage particles.ColE1

DNA segments required for plasmid

replication These plasmids are called as
cosmids.

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